The human estrogen receptor has two independent nonacidic transcriptional activation functions
Identifieur interne : 000397 ( France/Analysis ); précédent : 000396; suivant : 000398The human estrogen receptor has two independent nonacidic transcriptional activation functions
Auteurs : Laszio Tora [France] ; John White [France] ; Christel Brou [France] ; Dlane Tasset [France] ; Nicholas Webster [France] ; Elisabeth Scheer [France] ; Plerre Chambon [France]Source :
- Cell [ 0092-8674 ] ; 1989.
English descriptors
- Teeft :
- Aads, Acidic, Acidic activators, Activates transcription, Activation, Activation function, Activation functions, Activator, Activator expression vectors, Additive effects, Amino, Amino acids, Assay, Cell types, Chambon, Chicken embryo fibroblasts, Chimeric, Chimeric activators, Densitometric scanning, Embo, Embryo, Enhancer, Estrogen, Estrogen receptor, Experimental procedures, Expression vectors, Gal4, Glucocorticoid receptors, Hela, Hela cells, Heterosynergize, Homosynergism, Human estrogen receptor, Independent transfections, Internal control, Kumar, Minimal promoter, Minimal promoters, More transcription, Mpmlp, Nuclear receptors, Nuclease, Nuclease analyses, Nuclease analysis, Oestrogen, Petri dishes, Plasmid, Promoter, Ptashne, Receptor, Reporter gene, Reporter genes, Reporter plasmids, Synergistically, Synergize, Tata, Tata region, Tora, Transcription, Transcription factor, Transcriptional, Transfected, Transfections, Unpublished data.
Abstract
Abstract: We have previously reported the presence of a hormone-Inducible transcriptional activation function (TAF-2) within the region of the estrogen receptor (ER) that contains the hormone binding domain. We show here that the N-terminal A B region of the ER contains an Independent constitutive activation function (TAF-1) that exhibits cell type specificity since it activates transcription efficiently in chicken embryo fibroblasts, but only poorly in HeLa cells. By analyzing the ability of TAF-1, TAF-2, and the GAL4 and VP16 acidic activating domains (AADs) to homosynergize and heterosynergize with one another and with the factor binding to the upstream element (UE) of the adenovirus 2 major late promoter, we show that the activation properties of TAF-1 and TAF-2 are different and distinct from those of AADs, in agreement with the absence of acidic amino acid stretches in TAF-1 and TAF-2.
Url:
DOI: 10.1016/0092-8674(89)90031-7
Affiliations:
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sort="Webster, Nicholas" uniqKey="Webster N" first="Nicholas" last="Webster">Nicholas Webster</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS Unité 184 de Biologie Moléculaire et de Génie Génétique de I'INSERM Institut de Chimie Biologique</wicri:regionArea></affiliation></author><author><name sortKey="Scheer, Elisabeth" sort="Scheer, Elisabeth" uniqKey="Scheer E" first="Elisabeth" last="Scheer">Elisabeth Scheer</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS Unité 184 de Biologie Moléculaire et de Génie Génétique de I'INSERM Institut de Chimie Biologique</wicri:regionArea></affiliation></author><author><name sortKey="Chambon, Plerre" sort="Chambon, Plerre" uniqKey="Chambon P" first="Plerre" last="Chambon">Plerre Chambon</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS Unité 184 de Biologie Moléculaire et de Génie Génétique de I'INSERM Institut de Chimie Biologique</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Cell</title><title level="j" type="abbrev">CELL</title><idno type="ISSN">0092-8674</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1989">1989</date><biblScope unit="volume">59</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="477">477</biblScope><biblScope unit="page" to="487">487</biblScope></imprint><idno type="ISSN">0092-8674</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0092-8674</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Aads</term><term>Acidic</term><term>Acidic activators</term><term>Activates transcription</term><term>Activation</term><term>Activation function</term><term>Activation functions</term><term>Activator</term><term>Activator expression vectors</term><term>Additive effects</term><term>Amino</term><term>Amino acids</term><term>Assay</term><term>Cell types</term><term>Chambon</term><term>Chicken embryo fibroblasts</term><term>Chimeric</term><term>Chimeric activators</term><term>Densitometric scanning</term><term>Embo</term><term>Embryo</term><term>Enhancer</term><term>Estrogen</term><term>Estrogen receptor</term><term>Experimental procedures</term><term>Expression vectors</term><term>Gal4</term><term>Glucocorticoid receptors</term><term>Hela</term><term>Hela cells</term><term>Heterosynergize</term><term>Homosynergism</term><term>Human estrogen receptor</term><term>Independent transfections</term><term>Internal control</term><term>Kumar</term><term>Minimal promoter</term><term>Minimal promoters</term><term>More transcription</term><term>Mpmlp</term><term>Nuclear receptors</term><term>Nuclease</term><term>Nuclease analyses</term><term>Nuclease analysis</term><term>Oestrogen</term><term>Petri dishes</term><term>Plasmid</term><term>Promoter</term><term>Ptashne</term><term>Receptor</term><term>Reporter gene</term><term>Reporter genes</term><term>Reporter plasmids</term><term>Synergistically</term><term>Synergize</term><term>Tata</term><term>Tata region</term><term>Tora</term><term>Transcription</term><term>Transcription factor</term><term>Transcriptional</term><term>Transfected</term><term>Transfections</term><term>Unpublished data</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: We have previously reported the presence of a hormone-Inducible transcriptional activation function (TAF-2) within the region of the estrogen receptor (ER) that contains the hormone binding domain. We show here that the N-terminal A B region of the ER contains an Independent constitutive activation function (TAF-1) that exhibits cell type specificity since it activates transcription efficiently in chicken embryo fibroblasts, but only poorly in HeLa cells. By analyzing the ability of TAF-1, TAF-2, and the GAL4 and VP16 acidic activating domains (AADs) to homosynergize and heterosynergize with one another and with the factor binding to the upstream element (UE) of the adenovirus 2 major late promoter, we show that the activation properties of TAF-1 and TAF-2 are different and distinct from those of AADs, in agreement with the absence of acidic amino acid stretches in TAF-1 and TAF-2.</div></front></TEI><affiliations><list><country><li>France</li></country></list><tree><country name="France"><noRegion><name sortKey="Tora, Laszio" sort="Tora, Laszio" uniqKey="Tora L" first="Laszio" last="Tora">Laszio Tora</name></noRegion><name sortKey="Brou, Christel" sort="Brou, Christel" uniqKey="Brou C" first="Christel" last="Brou">Christel Brou</name><name sortKey="Chambon, Plerre" sort="Chambon, Plerre" uniqKey="Chambon P" first="Plerre" last="Chambon">Plerre Chambon</name><name sortKey="Scheer, Elisabeth" sort="Scheer, Elisabeth" uniqKey="Scheer E" first="Elisabeth" last="Scheer">Elisabeth Scheer</name><name sortKey="Tasset, Dlane" sort="Tasset, Dlane" uniqKey="Tasset D" first="Dlane" last="Tasset">Dlane Tasset</name><name sortKey="Webster, Nicholas" sort="Webster, Nicholas" uniqKey="Webster N" first="Nicholas" last="Webster">Nicholas Webster</name><name sortKey="White, John" sort="White, John" uniqKey="White J" first="John" last="White">John White</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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